The development of genome-editing technologies, mainly clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9), has transformed modern biotechnology, enabling precise modification of genetic material across diverse organisms. In the agri-food sector, CRISPR/Cas9 provides an accurate tool for genome modification of crop plants to increase their yield, stress tolerance, disease resistance and nutritional content. Unlike genetic modification methods used in genetically modified organisms (GMOs), CRISPR/Cas9 enables precise gene editing without introducing foreign DNA, sparking debates over its classification and regulation within the current GMO framework. Despite its significant potential, using CRISPR/Cas9 technology in the agri-food sector raises ethical, ecological, and socio-economic concerns that require careful evaluation and transparent governance. Recent European Union (EU) legislative measures seek to establish regulatory pathways that balance innovation with biosafety and public confidence. This brief report examines the benefits, challenges, and public concerns associated with the use of CRISPR/Cas9 technology in the agri-food industry, highlighting its role in advancing a sustainable and responsible food system.